Search results for "methods [Polymerase Chain Reaction]"
showing 10 items of 257 documents
Study of published articles on management accounting in Brazil and Spain
2013
En las últimas décadas, se han producido cambios importantes en la contabilidad de gestión con la inclusión de nuevos temas y métodos de investigación, revistas exclusivas y, en especial, estudios con perspectivas multidisciplinarias. Estos cambios se han detectado con estudios divulgados en publicaciones de revistas importantes. En este contexto surge la siguiente pregunta de investigación: ¿Cuál es el perfil de las investigaciones a respecto de la contabilidad de gestión en España y Brasil? Así, el objetivo del presente trabajo es identificar y analizar los temas y métodos de investigación aplicados en los estudios de contabilidad de gestión en España y Brasil, presentar cómo esas investi…
Gain-scheduled H-infinity observer design for nonlinear stochastic systems with time-delay and actuator saturation
2012
In this paper, we propose a method for designing continuous gain-scheduled robust H ∞ observer on a class of extended stochastic nonlinear systems subject to time delay and actuator saturation. Initially, gradient linearization procedure is applied to describe such extended nonlinear systems into several model-based linear systems. Next, a robust linear H ∞ observer is designed to such linear stochastic models. Subsequently, a convex hull set is investigated and sufficient condition is derived in terms of feedback observer to determine whether a given initial condition belongs to an ellipsoid invariant set. Finally, continuous gain-scheduled approach is employed to design continuous nonline…
Distribution Amplitudes of Heavy-Light Mesons
2019
A symmetry-preserving approach to the continuum bound-state problem in quantum field theory is used to calculate the masses, leptonic decay constants and light-front distribution amplitudes of empirically accessible heavy-light mesons. The inverse moment of the $B$-meson distribution is particularly important in treatments of exclusive $B$-decays using effective field theory and the factorisation formalism; and its value is therefore computed: $\lambda_B(\zeta = 2\,{\rm GeV}) = 0.54(3)\,$GeV. As an example and in anticipation of precision measurements at new-generation $B$-factories, the branching fraction for the rare $B\to \gamma(E_\gamma) \ell \nu_\ell$ radiative decay is also calculated…
Illumina-based RiboMethSeq approach for mapping of 2'-O-Me residues in RNA
2016
International audience; RNA 2'-O-methylation is one of the ubiquitous nucleotide modifications found in many RNA types from Bacteria, Archaea and Eukarya. RNAs bearing 2'-O-methylations show increased resistance to degradation and enhanced stability in helices. While the exact role of each 2'-O-Me residue remained elusive, the catalytic protein Fibrillarin (Nop1 in yeast) responsible for 2'-O-methylation in eukaryotes, is associated with human pathologies. Therefore, there is an urgent need to precisely map and quantify hundreds of 2'-O-Me residues in RNA using high-throughput technologies. Here, we develop a reliable protocol using alkaline fragmentation of total RNA coupled to a commonly …
SM identification of approximating models forH∞ robust control
1999
Set Membership (SM) W, identification of mixed parametric and nonparametric models is investigated, aimed to estimate a low order approximating model and an identification error, giving a measure of the unmodeled dynamics in a form well suited for H, control methodologies. In particular, the problem of estimating the parameters of the parametric part and the H, bound on the modeling error is solved using frequency domain data, supposing lbo bounded measurement errors and exponentially stable unmodeled dynamics. The effectiveness of the proposed procedure is tested on some numerical examples, showing the advantages of the proposed methods over the existing nonparametric H, identification app…
Biopsy vs. brushing: comparison of two sampling methods for the detection of HPV-DNA in squamous cell carcinoma of the oral cavity
2012
Background: HR HPV infection was proposed as aetiological factor of oral squamous cell carcinomas (OSCC). HPV frequency in OSCC is highly variable, due to the discrepancy in oral sampling procedures, HPV testing methods and inclusion criteria regarding tumour site (strictly oral cavity vs. nearby structures). Our aim was to compare HPV DNA frequency and type-specific distribution in paired cytological and histological samples of SCC strictly located in oral cavity. The correlation between HPV detection rate by each method of sampling and demographical, behavioural and clinical-pathological variables was also examined. Patients and methods: HPV DNA was detected in brushed cells and formalin-…
The contribution of colour and spatial frequency cues to contour integration
2011
Beobachtungen nach einer neuen optischen Methode: ein Beitrag zur Experimental-Physik
1864
Inhalt: Princip der Beobachtungs-Methode... 2 Specielle Beschreibung des Apparates... 8 Bemerkungen über den Gebrauch und die Adjustirung... 13 Schlieren in Gläsern ... 16 Beobachtungen mittels des Schlieren-Apparates... 18 Veränderung durchsichtiger Körper durch Temperatur und Druck... 19 Diffusionsbewegungen... 21 Untersuchung der Flammen... 23 Beugungsphänomene... 26 Sichtbarmachung der Schallwellen... 27 Beobachtungen über den electrischen Funken... 29 Der Inductionsfunken... 35 Der Flaschenfunken... 42 Schlussbemerkungen... 49
Direct measurement of the melting temperature of supported DNA by electrochemical method
2003
The development of biosensors based on DNA hybridization requires a more precise knowledge of the thermodynamics of the hybridization at a solid interface. In particular, the selectivity of hybridization can be affected by a lot of parameters such as the single-strand (ss)DNA density, the pH, the ionic strength or the temperature. The melting temperature, T(m), is in part a function of the ionic strength and of the temperature and therefore provides a useful variable in the control of the selectivity and sensitivity of a DNA chip. The electrochemical technique has been used to determine the T(m) values when the probe is tethered by a DNA self-assembled monolayer (SAM). We have built a speci…
La distribuzione di Maxwell-Boltzmann: i risultati di una sperimentazione svolta nell'ambito dei corsi di laurea in ingegneria
2011
This paper describes the outcomes of an teaching experiment conducted in the seminar held at the Faculty Engineering of Palermo University. The theme of the seminar focused on undergraduate experiment that yields the velocity distribution of thermionic electrons by analyzing the I-V characteristics of diodes and triodes. By using a simple model, the velocity distribution of thermionic electrons emitted by the vacuum tube cathode can be described by Maxwell's distribution. The experiment allows students to focus on the distribution function more than on difficulties arising from the complexity of thermionic emission. Questo articolo descrive i risultati di una sperimentazione didattica condo…